Changes in Pericellular Matrix Composition and Chondrocyte Morphology of Human Articular Cartilage in Osteoarthritis
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چکیده
INTRODUCTION: Structure and composition of articular cartilage as well as metabolic activity of chondrocytes (cartilage cells) change in osteoarthritis (OA) [1]. Among these changes, cell volume has been reported to increase [1,2]. Furthermore, the structure, composition and mechanical properties of the pericellular matrix (PCM) alter in OA [3]. However, the role of PCM in modulating volumetric and morphological changes of chondrocytes in OA is poorly known. Collagen and proteoglycan (PG) contents of the PCM in the deep zone of human OA articular cartilage have been clarified using Fourier Transform Infrared Imaging (FTIRI) [4]. However, changes in the composition of the PCM in the superficial zone of human cartilage during OA progression are currently unknown. In this study, morphology of the superficial zone chondrocytes was investigated in intact and degenerated human articular cartilage using light microscope. Further, FTIRI and digital densitometry (DD) were utilized to determine the PCM composition. We hypothesize that the collagen of the PCM is related to cell morphology in OA.
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